ABSTRACT
Berberis darwinii Hook es una especie que habita el sur de Chile y la Patagonia, utilizada por la etnia mapuche para el tratamiento de procesos inflamatorios, estados febriles y dolor de estomacal. El propósito del siguiente estudio fue evaluar in vitro las propiedades del extracto total y de alcaloides de raíz de B. darwinii sobre viabilidad celular y la translocación del factor nuclear NF-kB en línea celular RAW 264.7. Se observó que los extractos no afectan negativamente la viabilidad en las células e inhibieron la translocación del factor nuclear NF-kB asociado a la modulación de la inflamación solo frente al extracto total. Estos resultados indicarían que B. darwinii podría inhibir algunos mecanismos específicos de la defensa celular al modular la translocación de NF- kB.
Berberis darwinii Hook is a species that inhabits southern Chile and Patagonia, used by the Mapuche ethnic group for the treatment of inflammatory processes, febrile states and stomach pain. The purpose of the following study was to evaluate in vitro the properties of the total extract and alkaloids of the root of B. darwinii on cell viability and the translocation of the nuclear factor NF-kB in cell line RAW 264.7. It was observed that the extracts did not negatively affect the viability in the cells and inhibited the translocation of the nuclear factor NF-kB associated with the modulation of inflammation only against the total extract. These results indicate that B. darwinii could inhibit some specific mechanisms of cell defense by modulating the translocation of NF-kB.
Subject(s)
Plant Extracts/pharmacology , NF-kappa B/drug effects , Berberis , RAW 264.7 Cells/drug effects , Cell Survival/drug effects , Fluorescent Antibody Technique , Plant Roots , Methanol , Alkaloids/pharmacology , Flow Cytometry , Immunologic Factors/pharmacologyABSTRACT
In this study we investigated the anti-inflammatory effects and mechanisms of Hizikia fusiformis [HF] extracts in lipopolysaccharide [LPS]-induced RAW 264.7 cells. We extracted HF using solvent and sub-critical water techniques. In results, HF extracts inhibited nitric oxide [NO] production in cell-free and LPS-stimulated RAW 264.7 cells. HF210 [extract prepared with sub critical water at 210[degree]C] was most effective. The HF210 extract dose-dependently inhibited inducible nitric oxide synthase expression [iNOS] and nuclear factor kappa [NF-kB] p65 translocation from cytosol to the nucleus. Furthermore, HF210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase [p38 MAPK], Jun N-terminal kinase [JNK], and signal transducers and activators of transcription [STAT]-1in LPS-induced RAW 264.7 cells. Thus, our results suggest that anti-inflammatory effects of HF210 extract showed a noticeable distinction by regulation of multiple signaling pathways in LPS-induced RAW 264.7 cells